Publications

Díaz-Narváez, Atanasov KE, Murillo E, Alcázar R (2024) Pararhizobium sp. strains enhancing chilling stress tolerance and yield in tomato plants. Plant Stress 14: 100587

Zhang C., Atanasov KE, Murillo E, Vives-Peris V, Zhao J, Deng C, Gómez-Cadenas A, Alcázar R (2023) Spermine deficiency shifts the balance between jasmonic acid and salicylic acid-mediated defence responses in Arabidopsis. Plant, Cell & Environ.  46:3949-3970

Zhang C., Atanasov K.E., Alcázar R. (2023) Spermine inhibits PAMP-induced ROS and Ca 2+ burst and reshapes the transcriptional landscape of PTI in Arabidopsis. J Exp Bot. 74: 427-442

Panahirad S, Gohari G, Mahdavinia G, Jafari H, Kulak M, Fotopoulos V, Alcázar R, Dadpour M. (2023) Foliar application of chitosan-putrescine nanoparticles (CTS-Put NPs) alleviates cadmium toxicity in grapevine (Vitis vinifera L.) cv. Sultana: modulation of antioxidant and photosynthetic status. BMC Plant Biol. 23:411

Panahirad S, Dadpour M, Gohari G, Akbari A, Mahdavinia G, Jafari H, Kulak M, Alcázar R, Fotopoulos V. (2023) Putrescine-functionalized carbon quantum dot (put-CQD) nanoparticle: A promising stress-protecting agent against cadmium stress in grapevine (Vitis vinifera cv. Sultana). Plant Physiol Biochem. 97:107653

Atanasov K.E., Díaz-Narváez L., Alcázar R. (2022) Ammonium and nitric oxide condition the establishment of Arabidopsis Ler/Kas-2 immune-related hybrid incompatibility. Planta 256: 76

Platre MP, Satbhai SB, Brent L, Gleason MF, Cao M, Grison M, Glavier M, Zhang L, Gaillochet C, Goeschl C, Giovannetti M, Enugutti B, Neveu J, von Reth M, Alcázar R, Parker JE, Vert G, Bayer E, Busch W. (2022) The receptor kinase SRF3 coordinates iron-level and flagellin dependent defense and growth responses in plants. Nature Commun. 13: 4445

Zhang C., Atanasov K.E., Alcázar R (2022) Spermine inhibits pathogen-associated molecular pattern (PAMP) ROS and Ca2+ burst and reshapes the transcriptional landscape of PAMP-triggered immunity in Arabidopsis thaliana. Preprint BioRxiv doi: https://doi.org/10.1101/2022.05.05.490826

Faragó D; Zsigmond L; Benyó D; Alcázar R; Rigó G; Ayaydin F; Rabilu SA; Hunyadi‐Gulyás E; Szabados l (2022) Small paraquat resistance proteins modulate paraquat and ABA responses and confer drought tolerance to overexpressing Arabidopsis plants. Plant Cell & Environ. 45: 1985-2003. 

Liu, C., Alcázar, R. (2021) A new insight into the contribution of putrescine to defense in Arabidopsis thaliana. Plant Signal Behav. 16: 1885187

Alcázar R, Bueno M, Tiburcio AF (2020) Polyamines: small amines with large effects on plant abiotic stress tolerance. Cells 9:2373

Liu, C., Atanasov, K.E., Arafaty, N., Murillo, E., Tiburcio A.F., Zeier, J., Alcázar, R. (2020) Putrescine elicits ROS – dependent activation of the salicylic acid pathway in Arabidopsis thaliana. Plant, Cell & Environ. doi: 10.1111/pce.13874

Alcázar R, Fortes AM, Tiburcio AF. (2020) Editorial: Polyamines in plant biotechnology, food nutrition and human health. Front Plant Sci. 11:120

Liu, C., Atanasov, K.E., Tiburcio, A.F., Alcázar, R. (2019) The polyamine putrescine contributes to H2O2 and RbohD/F -dependent positive feedback loop in Arabidopsis PAMP triggered immunity. Front Plant Sci (10): 894 

Atanasov, K. E., Liu, C., Erban, A., Kopka, J., Parker, J. E., Alcázar, R. (2018). NLR mutations suppressing immune hybrid incompatibility and their effects on disease resistance. Plant Physiology, doi: 10.1104/pp.18.00462.

Alcázar and Tiburcio Eds. Polyamines. Methods and Protocols. Springer. Book.

Tiburcio AF, Alcázar R. Potential Applications of Polyamines in Agriculture and Plant Biotechnology. Methods Mol Biol. 2018;1694:489-508. doi: 10.1007/978-1-4939-7398-9_40. Review. PubMed PMID: 29080190.

Barboza-Barquero L, Esker P, Alcázar R. Genome-Wide Association Mapping Analyses Applied to Polyamines. Methods Mol Biol. 2018;1694:427-432. doi:10.1007/978-1-4939-7398-9_35. PubMed PMID: 29080185.

Atanasov KE, Liu C, Tiburcio AF, Alcázar R. Generation of EMS-Mutagenized Populations of Arabidopsis thaliana for Polyamine Genetics. Methods Mol Biol. 2018;1694:343-346. doi: 10.1007/978-1-4939-7398-9_29. PubMed PMID: 29080179.

Bitrián M, Tiburcio AF, Alcázar R. Determination of Posttranslational Modifications by 2D PAGE: Applications to Polyamines. Methods Mol Biol. 2018;1694:337-341. doi: 10.1007/978-1-4939-7398-9_28. PubMed PMID: 29080178.

Tiburcio AF, Alcázar R. Determination of S-Adenosylmethionine Decarboxylase Activity in Plants. Methods Mol Biol. 2018;1694:123-128. doi: 10.1007/978-1-4939-7398-9_12. PubMed PMID: 29080162.

Alcázar R, Tiburcio AF. Determination of Arginine and Ornithine Decarboxylase Activities in Plants. Methods Mol Biol. 2018;1694:117-122. doi: 10.1007/978-1-4939-7398-9_11. PubMed PMID: 29080161.

Lalaleo L, Alcázar R, Palazon J, Moyano E, Cusido RM, Bonfill M. Comparing aryltetralin lignan accumulation patterns in four biotechnological systems of Linum album. J Plant Physiol. 2018 Jun 15;228:197-207. doi: 10.1016/j.jplph.2018.06.006. [Epub ahead of print] PubMed PMID: 29960916.

Lalaleo L, Testillano P, Risueño MC, Cusidó RM, Palazon J, Alcázar R, Bonfill M. Effect of in vitro morphogenesis on the production of podophyllotoxin derivatives in callus cultures of Linum album. J Plant Physiol. 2018 May 18;228:47-58. doi: 10.1016/j.jplph.2018.05.007. [Epub ahead of print] PubMed PMID: 29852334.

Zarza X, Atanasov KE, Marco F, Arbona V, Carrasco P, Kopka J, Fotopoulos V, Munnik T, Gómez-Cadenas A, Tiburcio AF, Alcázar R. (2017) Polyamine Oxidase 5 loss-of-function mutations in Arabidopsis thaliana trigger metabolic and transcriptional reprogramming and promote salt stress tolerance. Plant Cell & Environ. 40: 527-542.

Sequera-Mutiozabal M, Antoniou C, Tiburcio AF, Alcázar R, Fotopoulos V. (2017) Polyamines: Emerging hubs promoting drought and salt stress tolerance in plants. Curr Mol Biol Rep. 1:28-36

Treves H, Murik O, Kedem I, Eisenstadt D, Meir S, Rogachev I, Szymanski J, Keren N, Orf I, Tiburcio AF, Alcázar R, Aharoni A, Kopka J, Kaplan A. (2017) Metabolic flexibility underpins growth capabilities of the fastest growing alga. Curr. Biol. 27:2559-2567

Ariga H, Katori T, Tsuchimatsu T, Hirase T, Tajima Y, Parker JE, Alcázar R, Koornneef M, Hoekenga O, Lipka AE, Gore MA, Sakakibara H, Kojima M, Kobayashi Y, Iuchi S, Kobayashi M, Shinozaki K, Sakata Y, Hayashi T, Saijo Y, Taji T. (2017) NLR locus-mediated trade-off between abiotic and biotic stress adaptation in Arabidopsis. Nature Plants 3:17072

Atanasov KE, Barboza-Barquero L, Tiburcio AF, Alcázar R (2016) Genome wide association mapping for the tolerance to the polyamine oxidase inhibitor guazatine in Arabidopsis thaliana. Front. Plant Sci.7:40

Sequera-Mutiozabal MI, Erban A, Kopka J, Atanasov K, Bastida J, Fotopoulos V, Alcázar R and Tiburcio AF (2016). Global metabolic profiling of Arabidopsis Polyamine Oxidase 4 (AtPAO4) loss-of-function mutants exhibiting delayed dark-induced senescence. Front. Plant Sci. 7:173.

Stuttmann J, Peine N, Garcia AV, Wagner C, Choudhury SR, Wang Y, James GV, Griebel T, Alcázar R, Tsuda K, Schneeberger K, Parker JE (2016) Arabidopsis thaliana DM2h (R8) within the Landsberg RPP1-like Resistance locus underlies three different cases of EDS1-conditioned autoimmunity.PLoS Genet.12 :e1005990

Marco F, Bitrián M, Carrasco P, Rajam MV, Alcázar R, Tiburcio AF. Genetic Engineering Strategies for Abiotic Stress Tolerance in Plants. In: Plant Biology and Biotechnology Vol.2. Springer 2015. pp:579-610.

Alcázar R, von Reth M, Bautor J, Chae E, Weigel D, Koornneef M and Parker JE. (2014) Analysis of a plant complex Resistance gene locus underlying immune-related hybrid incompatibility and its occurrence in nature. PLoS Genet 10(12): e1004848.

Alcázar R and Tiburcio AF (2014) Plant polyamines in stress and development: an emerging area of research in plant sciences. Front. Plant Sci. 5:319

Tiburcio AF, Altabella T, Bitrián M and Alcázar R. (2014) The Roles of Polyamines during the lifespan of plants:from development to stress. Planta 240:1-18.

 Alcazar, R.; Tiburcio, A. F. (2014) Polyamines in stress protection - applications in agriculture in Plant adaptation to environmental change: significance of amino acids and their derivatives Pages: 129-140. CABI; Wallingford; UK. ISBN: 978-1-78064-273-4.

Barboza L, Effgen S, Alonso-Blanco C, Kooke R, Keurentjes J, Koornneef M, Alcázar R. (2013) Arabidopsis semi-dwarfs evolved from independent mutations in GA20ox1, orthologue to green revolution dwarf alleles in rice and barley. PNAS 110:15818-23.

Alcázar R, Pecinka A, Aarts MG, Fransz PF, Koornneef M. Signals of speciation within Arabidopsis thaliana in comparison with its relatives. Curr Opin Plant Biol. 2012 15(2):205-11.

Bitrián M, Zarza X, Altabella T, Tiburcio AF, Alcázar R. Polyamines under stress: metabolic crossroads and hormonal crosstalks. Metabolites (new  journal). Metabolites. 2012; 2(3):516-528  

Alcázar R, Parker JE. The impact of temperature on balancing immune responsiveness and growth in Arabidopsis. Trends Plant Sci. 2011 Dec;16(12):666-75.

Alcázar R, Reymond M, Schmitz G, de Meaux J. Genetic and evolutionary perspectives on the interplay between plant immunity and development. Curr Opin Plant Biol. 2011 Aug;14(4):378-84.

Marco F, Alcázar R, Tiburcio AF, Carrasco P. Interactions between polyamines and abiotic stress pathway responses unraveled by transcriptome analysis of polyamine overproducers. OMICS. 2011 Nov;15(11):775-81.

Alcázar R, Cuevas JC, Planas J, Zarza X, Bortolotti C, Carrasco P, Salinas J, Tiburcio AF, Altabella T. Integration of polyamines in the cold acclimation response. Plant Sci. 2011 Jan;180(1):31-8.

Alet AI, Sánchez DH, Ferrando A, Tiburcio AF, Alcazar R, Cuevas JC, Altabella T, Pico FM, Carrasco-Sorli P, Menéndez AB, Ruiz OA. Homeostatic control of polyamine levels under long-term salt stress in Arabidopsis: changes in putrescine content do not alleviate ionic toxicity. Plant Signal Behav. 2011 Feb;6(2):237-42.

Alcázar R, Bitrián M, Bartels D, Koncz C, Altabella T, Tiburcio AF. Polyamine metabolic canalization in response to drought stress in Arabidopsis and the resurrection plant Craterostigma plantagineum. Plant Signal Behav. 2011 Feb;6(2):243-50.

Alcázar R, García AV, Kronholm I, de Meaux J, Koornneef M, Parker JE, Reymond M. Natural variation at Strubbelig Receptor Kinase 3 drives immune-triggered incompatibilities between Arabidopsis thaliana accessions. Nat Genet. 2010 Dec;42(12):1135-9.

Alcázar R, Altabella T, Marco F, Bortolotti C, Reymond M, Koncz C, Carrasco P, Tiburcio AF. Polyamines: molecules with regulatory functions in plant abiotic stress tolerance. Planta. 2010 May;231(6):1237-49.

Alcázar R, Planas J, Saxena T, Zarza X, Bortolotti C, Cuevas J, Bitrián M, Tiburcio AF, Altabella T. Putrescine accumulation confers drought tolerance in transgenic Arabidopsis plants over-expressing the homologous Arginine decarboxylase 2 gene. Plant Physiol Biochem. 2010 Jul;48(7):547-52.

Cuevas JC, López-Cobollo R, Alcázar R, Zarza X, Koncz C, Altabella T, Salinas J, Tiburcio AF, Ferrando A. Putrescine as a signal to modulate the indispensable ABA increase under cold stress. Plant Signal Behav. 2009 Mar;4(3):219-20.

Alcázar R, García AV, Parker JE, Reymond M. Incremental steps toward incompatibility revealed by Arabidopsis epistatic interactions modulating salicylic acid pathway activation. Proc Natl Acad Sci U S A. 2009 Jan 6;106(1):334-9.

 Berdasco M, Alcázar R, García-Ortiz MV, Ballestar E, Fernández AF, Roldán-Arjona T, Tiburcio AF, Altabella T, Buisine N, Quesneville H, Baudry A, Lepiniec L, Alaminos M, Rodríguez R, Lloyd A, Colot V, Bender J, Canal MJ, Esteller M, Fraga MF. Promoter DNA hypermethylation and gene repression in undifferentiated Arabidopsis cells. PLoS One. 2008 Oct 1;3(10):e3306.

Cuevas JC, López-Cobollo R, Alcázar R, Zarza X, Koncz C, Altabella T, Salinas J, Tiburcio AF, Ferrando A. Putrescine is involved in Arabidopsis freezing tolerance and cold acclimation by regulating abscisic acid levels in response to low temperature. Plant Physiol. 2008 Oct;148(2):1094-105.

Alcázar R, Marco F, Cuevas JC, Patron M, Ferrando A, Carrasco P, Tiburcio AF, Altabella T. Involvement of polyamines in plant response to abiotic stress. Biotechnol Lett. 2006 Dec;28(23):1867-76.

Alcázar R, García-Martínez JL, Cuevas JC, Tiburcio AF, Altabella T. Overexpression of ADC2 in Arabidopsis induces dwarfism and late-flowering through GA deficiency. Plant J. 2005 Aug;43(3):425-36.

Bortolotti C, Cordeiro A, Alcázar R, Borrell A, Culiañez-Macià FA, Tiburcio AF, Altabella T. Localization of arginine decarboxylase in tobacco plants. Physiol Plant. 2004 Jan;120(1):84-92.

Panicot M, Minguet EG, Ferrando A, Alcázar R, Blázquez MA, Carbonell J, Altabella T, Koncz C, Tiburcio AF. A polyamine metabolon involving aminopropyl transferase complexes in Arabidopsis. Plant Cell. 2002 Oct;14(10):2539-51.